To our knowledge this is the first report of Johrei therapy—a complementary and alternative form of therapy—to be associated with improvement in objective measures of sleep. The exploratory aim of the study was to determine the effect of Johrei on sleep as measured by tissue markers in the brain when compared to control mice. Mice were divided into several groups. Those that were subjected to sleep interruption and also received Johrei recovered better than those subjected to sleep interruption alone. This study may have increased validity because it eliminates the possibility of the placebo effect demonstrated in human subjects.

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